Cyclin d1 clone sp4 flow cytometry
WebJan 15, 2024 · Transfection efficiency was determined by flow cytometry using a plasmid encoding the enhanced green fluorescent protein eGFP (Amaxa Biosystems). ... Purified monoclonal antibodies to cyclin D1 (clone SP4, RM-910), cyclin D3 (clone DCS-22, MS-215-R7) all from NeoMarkers, Fremont, CA and cyclin D2 (10R-C149a) from Fitzgerald … WebAnti-Cyclin D1 antibody [SP4] (ab16663) at 1/200 dilution + Wild-type HeLa cell lysate at 20 µg Performed under reducing conditions. Predicted band size: 33 kDa Observed band …
Cyclin d1 clone sp4 flow cytometry
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WebFlow cytometry All the peripheral blood samples (n=24) and some spleen cell suspensions (n=17) were stained using combinations of two or three monoclonal antibodies directly conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE) or … WebFlow Cytometry. NEW Reagents; Antibody Reagents; Instrument Setup & Sample Prep; Counting Beads; Industry-specific Testing. NEW Reagents; Reagents; Cleaning & …
WebAug 19, 2010 · Cyclin D1 is a protein involved in cell cycle regulation and is responsible for the cell’s proceeding through G 1 phase. For this reason, it has been considered that its … WebClone G124-326 recognizes human Cyclin D1. It does not cross-react with human cyclins D2 and D3. Recombinant full-length human Cyclin D1 was used as immunogen. ... (Clone-specific: Flow cytometry). Gong J, Traganos F, Darzynkiewicz Z. Threshold expression of cyclin E but not D type cyclins characterizes normal and tumour cells entering S phase ...
WebWe developed a flow cytometry method to detect cyclin D1 overexpression using the monoclonal antibody (MoAb) 5D4, and characterised its frequency in 93 B-cell … WebSep 1, 2011 · In our series, cyclin D1 showed similar sensitivity (96%) to annexin-1 and aberrantly stained only 1 case of MZL (cytogenetic analysis of the splenectomy specimen in this case was negative for t (11;14) and lacked all of the morphologic and genetic characteristics of mantle cell lymphoma).
Web• Description: Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a …
WebCCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. liberty christian church henderson ncWebHuman Cyclins Staining Procedure for Flow Cytometric Detection of Human Cyclins This is a standard protocol used at Pharmingen for Quality Control testing of the anti-cyclin … mcgraw edison galleon wallWebCyclin D1 / bcl-1 Ab-1 (Clone DCS-6) Mouse Monoclonal Antibody Cat. #MS-210-P0, -P1, ... • Flow Cytometry • Immunofluorescence ... (Ab 1-2µg/ml for 2hrs at RT) • Immunohistology (Not Suitable; Use Cyclin D1 Clone SP4) The optimal dilution for a specific application should be determined by the investigator. Cellular ... liberty christian center killeen tx 76543WebConcentration Unitmg/mLConcentration1Quantity0.1 mgVolume0.1 mLImmunogenPurified cyclin D1 proteinBackground InformationCyclin D1 (PRAD1, Bcl-1) is a… Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL Immunogen Purified cyclin D1 protein Background… mcgraw-edison gwc galleon wallWebCyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). Host Species: Rabbit. Clone: SP4. Isotype: IgG liberty christian church indianamcgraw davisson stewart tulsaWebcyclin D1 protein Applications and Suggested Dilutions: • Biological Blockade1 (Order Ab without azide) • Flow Cytometry • Immunofluorescence • Immunoprecipitation (Co … liberty christian church havelock