Cyto-id staining
WebCyto-ID labeling and Flow cytometry: HEK-293 cells were harvested in Accutase solution (Merck Millipore, SF006), rinsed in DPBS and incubated for 30 min at room temperature in DMEM without phenol red, containing 5% fetal bovine serum and the fluorescent Cyto-ID probe (Cyto-ID® Autophagy detection kit; Enzo Life Sciences, ENZ-51031K200). Webtook advantage of Cyto-ID, a uorescent dye for selective staining of autophagosomes [45–47], in two ways: First, we conducted double-labeling ow cytometry with Cyto-ID and the live-cell-permeant DNA marker DRAQ5, allowing for simultaneous monitoring of autophagy and cell cycle phases. Second, we developed a Cyto-ID-based ow-cyto-
Cyto-id staining
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WebJan 30, 2013 · Determination of autophagy (Cyto-ID staining) Flow cytometric analysis of Cyto-ID Green Detection Reagent stained cells was performed according to manufacturer's protocol (Cyto-ID Autophagy Detection Kit, ENZ-51031-K200, Enzo Life Sciences). In brief, harvested cells were washed with PBS, stained with Cyto-ID in indicator-free medium … WebPrime Juice Preloading Fluorescent Stain: 1 ml: LD003-0500: Novel Green Plus (20,000X) DNA Staining Reagent: 500 μl: AGT002-0500: Agarose Tablets, 110 pcs: 0.5g x 110: MB755-0100: ... 【CYTO-ID ®自噬檢測試劑盒 】 Autophagy detection kit貨號ENZ-51031-K200 最新產品 NEW PRODUCTS. 病毒基因組核酸分離試劑盒( Magnetic ...
WebThe cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO … WebOct 20, 2016 · 3.3 Cyto-ID Staining 1. Resuspend cell pellets in 0.25 mL phenol red-free RPMI medium containing 5 % FBS. 2. All the following steps are performed in the dark ( see Note 4 ). 3. Add 0.25 mL diluted Cyto-ID ® working solution to each tube and mix well ( see Note 7 ). 4. Incubate in a cell incubator for 30 min at 37 °C and 5 % CO 2. 5.
WebApr 14, 2024 · For CYTO-ID staining, cells were incubated for 30 min in N2B27 containing 2 μl/ml of CYTO-ID Green. Cells were then washed and fixed with 4% paraformaldehyde and processed for immunofluorescence imaging as above. Wide-field microscopes used: Leica DMRD upright fluorescence microscope fitted with a Leica DFC450 C camera 5 … WebMar 3, 2024 · The CYTO-ID dye is known to stain autophagic vesicles ( 24 ), and we have previously demonstrated similar results obtained by analyses of CYTO-ID staining and by detection of LC3-II ( 8 ). Forskolin enhanced the IR-induced autophagy, as revealed both by CYTO-ID staining and LC3-II detection ( Fig. 1A – D ).
WebFeb 10, 2016 · For Cyto-ID staining, cells were first stained for surface markers, Gr1 and CD11b, followed by staining with Cyto-ID for 30 min at 37°C, per the manufacturer’s protocol (Enzo Life Sciences, Farmingdale, NY, USA). Samples were run on a Cyan ADP flow cytometer and analyzed using Summit 4.3 software (Beckman Coulter, Brea, CA, …
WebIn this work, the Cyto-ID dye is shown to colocalize with RFP-LC3 in starved HeLa cells, which further validate the specificity of the dye. The developed image-based autophagy … grants for small business minorities in texasWebFeb 7, 2024 · (E) Cyto-ID staining of OV202 NTC and Sh1 cells in control, EBSS and EBSS + Baf treated cells. Cells were grown in either regular media or EBSS to induce autophagy. Cells were grown in either ... chipmunk laugh 1 hourWebNov 5, 2024 · After staining with Cyto-ID, autophagosomes were quantified measuring an increase in green fluorescence (FL1) using flow cytometry (** p < 0.01, ***p < 0.001 compared to the serum only group). chipmunk lane wolfeboro nhhttp://www.biovector.net/product/1777094.html chipmunk laughingWebCYTO-ID ® Autophagy Detection Kit measures autophagic vacuoles and monitors autophagic flux in live cells using a novel dye that selectively labels autophagic vacuoles. … grants for small business hawaiiWebJan 3, 2024 · Cyto-ID staining flow cytometry was used to detect autophagy compartments induced by autophagy activators with or without CQ (60 nM) pretreatment ( P < 0.05 by t test). e Cyto-ID fluorescence intensities of DMSO, EBSS, RAPA, and GDC-0152 Full size image Autophagy promotes GDC-0152-induced apoptosis in HL-60 cells grants for small business in ontarioWebCyto-Last™ Buffer is specially formulated for the storage of cytokine producing cells. When used, staining of intracellular and/or extracellular targets for flow cytometric analysis can be delayed for up to two weeks. Cells should be stored at 4°C during this time. This unique buffer ensures cells maintain a background staining signal equal ... chipmunk laugh