Toyobo ligation high ver 2
WebAug 25, 2024 · Introduction: Diabetes mellitus results in high rates of cardiovascular disease, such as microcirculation disorder of the lower limbs, with angiogenesis impairment being the main factor. The endothelium functions as a barrier between blood and the vessel wall. Vascular endothelial cell dysfunction caused by hyperglycemia is the main factor … WebThis kit includes the following components for 250 reactions, 20 μL total reaction volume. All reagents should be stored at -20°C. < HSTTX-101 >. 2x Buffer for rTth/ TTx (DNA) 1.25 mL x 2. Hot Start TTx DNA Polymerase (4U/ μL) 62.5 μL. Note: 2x Reaction Buffer contains essential components for the reaction (buffer, salts, Mg2+, dATP, dCTP ...
Toyobo ligation high ver 2
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Web本试剂是以T4 DNA Ligase为主体的高效率单管型连接试剂，广泛应用于各种末端DNA的连接反应。通过对原产品进行改良，可简便地用于各种用途。本试剂是在原产品『Ligation … WebフラグメントA およびフラグメントBそれぞれ200ng、ライゲーション試薬Ligation high Ver.2 (東洋紡)をトータル10μL となるように混合し、16℃で4 時間（または4℃、一晩） ... E-mail : [email protected] 東洋紡株式会社 バイオプロダクト営業部 (東京) …
WebTOYOBO USA INC LIGATION HIGH VER. 2. Manufacturer: TOYOBO USA INC LGK201. This product was recently added by customer request, and is available for your convenience. Web*2 Adding an equal volume of Ligation high Ver.2 to the DNA solution is recommended in the case of TA cloning. *3 The reaction can be prolonged for up to 2 hr. Standard cohesive …
WebThe template plasmid was digested using DpnI, and the PCR product was self- ligated using Ligation High with T4 Polynucleotide Kinase (Toyobo). E. coli DH5α were transformed with the ligated product, and the plasmid was pur- ified as described above. WebPrice from $9.99 to $1999.99 ligation high ver 2 ligase - by Bioz Stars , 2024-03 86 / 100 stars Images ligation high ver 2 ( Toyobo ) About News Press Release Team Advisors …
WebLigation high Ver.2 - Toyobo. EN. English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi …
WebJul 11, 2024 · The amplified fragment was ligated into the EcoRI and XhoI sites of the pENTR2B-dual entry vector (Life Technologies) using a DNA ligation kit (Ligation High ver.2, TOYOBO). CDS was cloned into pMDC45 or pMDC85 vectors (Curtis and Grossniklaus 2003 ) using LR clonase (Life Technologies). bolivian corn breadWebJul 1, 2024 · The ligation mixture was used to transform XL10-Gold competent cells and cultivated on a Luria-Bertani medium (10 g/L tryptone, 5 g/L yeast extract, and 10 g/L sodium chloride) agar plate containing 100 μg/mL of ampicillin, and 1% … bolivian coup teslaWebApr 13, 2024 · Plasmids were constructed either by ligation (Ligation high Ver.2, TOYOBO) or homologues recombination (In-Fusion HD Cloning Kit, Takara Bio) of DNA fragments using Escherichia coli DH5α competent cells (Competent Quick DH5α, TOYOBO). glycerin vitamin shoppeWeb3 [1] Insert insertion ligation 1. Method Five μl of DNA solution was prepared by adding 1,444 bp fragments (5-125 ng, 5-125 fmol) of pUC18/Taq I to dephosphorated pBluescript II/Ban … glycerin vocbolivia nc to orlando flWeb実験法としては、まず、λDNAの500bpをターゲットにrTaq DNA PolymeraseでPCRを行い、その反応液1μL (約6.4ng)と2μLのTベクター (50ng)を3μlのLigation highに加え、16℃に … bolivia nc to charleston scWebOct 18, 2024 · Add 5 μL Ligation high ver.2 (Toyobo). 4. Incubate for 1 h at 16 °C (or 4 °C for overnight), and stop the reaction by incubating for 10 min at 65 °C. 5. Separate the assembled ssDNA by 8 M urea-6% PAGE (see Note 3). 6. Stain the gel by SYBR Gold, and cut out the band of the expected size using clean scalpel and forceps. 7. glycerin vs alcohol extracts